New transcriptomic insights in two RNAi wheat lines with the gliadins strongly down-regulated by two endosperm specific promoters

The gluten proteins of wheat grain are responsible for visco-elastic properties flour, but they also trigger the immune-response celiac disease. In this work, two low-gliadin RNA interference (RNAi) lines that differ promoter driving silencing (D-hordein and γ-gliadin promoters D783 D793 lines, respectively), were characterized at transcriptomic, protein fraction levels in grain. Silencing gliadins provides a readjustment fractions affects to non-gluten (NGP), which increased both RNAi lines. Determination by means R5 monoclonal antibody showed strong reduction content Moreover, fructans, an oligosaccharide linked with development non-celiac sensitivity (NCWS) significantly decreased down-regulation impacts other metabolic processes, particularly on carbohydrate metabolism, enzyme regulator activity response stress. Genes transcription factors regulated ABA up-regulated, could suggest implication phytohormone stress observed